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Our research group recently published a paper entitled “genome analyses of single human oocytes” in CELL journal
Date:2015-01-05 Hits:1431

Single-cell genome analyses of human oocytes are important for meiosis research and preimplantation genomic screening in in-vitro fertilization (IVF). However, the non-uniformity of single-cell whole-genome amplification hindered its use. Our research group, leaded by Prof. Jie Qiao, Prof. Xiaoliang Xie and Prof. Fuchou Tang in peking university, first accomplishes genome analyses of single polar bodies. By sequencing the triads of the first and second polar bodies (PB1 and PB2) and the oocyte pronuclei from same female egg donors, we phase the genomes of these donors with detected SNPs and determine the crossover maps of their oocytes. Furthermore, the genome of the oocyte pronucleus, including information regarding aneuploidy and SNPs in disease-associated alleles, can be accurately deduced from the genomes of PB1 and PB2. The MALBAC-based preimplantation genomic screening in in-vitro fertilization (IVF) enables accurate and cost-effective preimplantation genomic screening (PGS) and selection of normal fertilized eggs for embryo transfer.